Bacterial transformation lab (firefly gene and ecoli) with guest Marta de Menezes (March 3)

We were in the lab this week learning the protocol for bacterial transformation. Marta de Menezes was on hand to participate and coach students through the process. Notes from the events will be posted soon. For now, here are some photos. (Yes, it was fun)

Sok leads the lab and explains aspetic technique

Sok leads the lab and explains aspetic technique

Tools of the trade

Tools of the trade

Preparing the membrane substrate

Preparing the membrane substrate

Sketching with the pipette before drawing with bacteria

Sketching with the pipette before drawing with bacteria

Drawing with ecoli in the agar petri dish

Drawing with ecoli in the agar petri dish

longshotdrawing

Puting the bacteria in the incubator (for 24 hours)

Puting the bacteria in the incubator (for 24 hours)

24 hours later:

Drawing #1, successful transformation!

Drawing #1, successful transformation! Photo: Richard Baldwin

Drawing #2

Drawing #2 Photo: Richard Baldwin

Drawing #3

Drawing #3 Photo: Richard Baldwin

The final results:

Photo: Rich Baldwin, Kendra Basenger, Claire Kenway, Tagny Duff, Jhave Johnson, Marta de Menezes, Alison loader, Genevieve Ruest

In photo: Rich Baldwin, Kendra Basenger, Claire Kenway, Tagny Duff, Jhave Johnson, Marta de Menezes, Alison loader, Genevieve Ruest

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